Chim3 prevents HIV-1 Vif-induced cell-cycle arrest in G₂. CEM A3.01 cells transduced with empty-LV or Chim3-LV (target cells) were synchronized with aphidicolin and then infected in a single-round infection with VSV-G–pseudotyped Vif-proficient (A-D) and Vif-deficient (Δvif; E-H) NL4-3–based HIV-1 molecular clones produced from either HEK-293T cells expressing empty-LV or Chim3-LV (producer cells). After intracellular p24Gag staining, PI content was evaluated only in electronically gated p24Gag⁺ cells. The G₂/G₁ ratio values are indicated in the top right corner of each cell-cycle profile panel.