Iron overload in the absence of STAT5A/B. Iron content and hepcidin expression in control and Stat5a/b^{f/f; TC} mice (A-E) and in mice that received a transplant of control or Stat5a/b^−/− fetal liver cells (F-I). (A) Non–heme liver iron in adult and neonate female control and Stat5a/b^{f/f; TC} mice. n = 5 neonates; n = 6 adults. (B,C) Serum iron (B) and transferrin saturation (C) in adult female control and Stat5a/b^{f/f; TC} mice. n = 12 in each group. (D) Hepcidin mRNA expression in neonate and adult control and Stat5a/b^{f/f; TC} mice (males and females). Hepcidin expression in control neonate and adult mice is set to 1. n = 9 in each group. (E) Perls Prussian blue iron staining of liver paraffin sections from adult female control and Stat5a/b^{f/f; TC} mice. Original magnification, ×100 in top panel and ×200 in bottom panel. Non–heme iron is stained blue. (F) Non–heme liver iron. (G) Serum iron. (H) Serum transferrin saturation. (I) Hepcidin mRNA expression. Hepcidin expression in mice that received a transplant of control cells is set to 1. n = 5 in each group for all the measurements. *P < .05 compared with control samples. P values were determined by 2-tailed unpaired t test. Error bars represent SEM.