M33-Meis1 confers growth inhibition on a myeloid progenitor cell line and to normal hematopoietic cells. (A) In vitro growth kinetics of indicated FACS-sorted cells plated in standard growth conditions. The fold changes compared with ND13+MIY are indicated. (B) In vitro cell expansion of unsorted transduced cells expressed as double-positive GFP⁺ and YFP⁺ cells over time. (A-B) The data are expressed as the mean ± SD of 3 independent transductions. (C) Peripheral blood chimerism as measured by FACS analysis. Data are plotted as the percentage (mean ± SD) of GFP⁺ and YFP⁺ cells. Gene transfer percentage refers to the proportion of transduced BM cells in the transplanted inoculum. (D) Kaplan-Meier survival curves for mice that received a transplant with BM transduced with the indicated gene(s). Statistical significance was determined by the log-rank test. (E) Peripheral blood chimerism of mice that received a transplant with MIY and M33-Meis1–transduced primary BM as measured by FACS analysis. Data are plotted as the percentage of circulating YFP⁺ cells for each mouse.