DHG inhibition of thrombin generation triggered by excess TF in mock- or HCII-depleted pooled plasma. Thrombin generation was initiated with 4 pM human TF, 8.3 μM PC:PS vesicles, and 40 μg/mL CTI (plasma concentrations) in mock- (A) or HCII-depleted plasma (B) in the presence of increasing DHG: 0 μM (●), 0.1 μM (○), 0.25 μM (■), 0.5 μM (□), 1 μM (▴), 2.5 μM (▵), 5 μM (♦), 10 μM (◇). Control reactions without TF or DHG are presented (▾). The time course of thrombin generation was measured as described in the “Fluorogenic assay for detection of plasma thrombin generation.” Thrombin generation curves represent the mean thrombin concentration over the first 30 minutes from replicate determinations (n = 3) and are identified by representative points. The relative velocity index for thrombin generation was plotted versus DHG concentration for each condition, and the data were fit as described in “Fluorogenic assay for detection of plasma thrombin generation” to determine the EC₅₀ for inhibition (C). Representative curves are presented.