Up-regulation of gene expression in patients after in vivo restimulation. The gp100-specific TCR transgene RNA level was corrected for the DNA level at each time interval and normalized to the infusion values (INF). RNA expression of β-actin, the endogenous TCR α-chain, and CD3ϵ before (PRE) and at time intervals after cell infusion was normalized to the infusion value (INF). (A) Patients A and B from the first cohort of this trial received IL-2 and peptide vaccination on the day of adoptive cell transfer. (B) Patients C through H from the second cohort of this trial received IL-2 with recombinant fowlpox vaccination on the day of adoptive cell transfer, as well as repeat administration of IL-2 with recombinant fowlpox vaccination approximately 1 month after adoptive cell transfer (indicated by ).