Multipotent hematopoietic progenitor persists in the absence of gp96. (A top) Dump gating of BM cells from WT and KO BM chimeras 1 month PTD. (Bottom) Gating of LSK (L) and PROG (P) populations. Numbers represent percentage of cells in the gated population. (B) Intracellular staining of gp96 on BM LSK and PROG populations from WT (solid line) and KO (dotted line) BM chimeras 2 months PTD using polyclonal gp96 antibody or isotype control (shaded). (C) CD49d, CD11a, and TLR2 expression on BM LSK populations from WT (solid line) and KO (dotted line) BM chimeras 1 month PTD. (D) Absolute numbers of BM LSK and PROG from WT (n = 3, □) and KO (n = 4, ■) BM chimeras 1 month and 7 months PTD. *P < .05. (E) LSK and PROG analysis from WT and KO BM chimeras 2 months PTD in the indicated tissues. (F) FLT3 expression on LSK from WT and KO BM chimeras 2 months PTD in the indicated tissues. Data are representative of at least 2 independent experiments.