De novo lymphopoiesis, but not myelopoiesis, is dependent on gp96. (A) Diagram of experimental design: BM cells from KO (7 months PTD BM chimera, 45.2) and WT (45.1) mice were transplanted in 20:1 ratio to lethally irradiated recipients (45.1) and analyzed 4 to 6 weeks PTD. (B) Representative congenic analysis of donor BM after 20:1 mix of WT and KO cells. (C) Congenic analysis of a representative recipient for relative contribution by WT (n = 4) and KO (n = 4) cells to the various hematopoietic tissues 4 to 6 weeks after BM reconstitution. (D-E) Congenic gated WT (gray) and KO (black) cells were analyzed for their relative contribution (number indicates percentage) to various lineages and hematopoietic tissues. (F) Percentage of B220⁺ cells over Gr1⁺ cells in BM and spleen of WT and KO mice. Data are representative of 2 independent experiments. *P < .05.