B- and T-cell lymphopoiesis is dependent on gp96 at critical transitional stages. (A) B220/IgM surface staining of BM cells from WT and KO BM chimeras 1 month PTD. (B) Absolute numbers of BM B cells in various fractions indicated in panel A from WT (n = 5, □) and KO (n = 6, ■) BM chimeras 3 to 4 weeks PTD. Data are pooled from 2 independent experiments. (C) CD43/CD24 surface staining on B220⁺ cells in BM. (D) Expression of λ5 pre-BCR and IL-7Rα on the surface of WT (solid line) and KO (dotted line) pre–B-cell line. Shaded histograms represent isotype control. (E) CD4/CD8 (top) surface staining and surface expression of CD49d (bottom) on thymocytes from WT and KO BM chimeras 1 month PTD. (F) DN gating (top) and CD44/CD25 surface staining on DN thymocytes from WT and KO BM chimeras 1 month PTD. (G) Absolute cell numbers of thymocytes from the indicated stages from WT (n = 3, □) and KO (n = 4, ■) BM chimeras 1 month and 7 months PTD. Data are representative of at least 2 independent experiments. *P < .05.