American Society of Hematology

Figure 4

$Figure 4. Chronic ethanol treatment leads to a suppression of global translation. (A) Raji and MCF-7 cells were treated with ethanol (0 or 20 mM) for 10 days. On the 10th day, cytoplasmic lysates were prepared and fractionated over sucrose gradients. From left to right, the distribution of free mRNA (not bound to ribosomes or ribosome subunits), ribosome subunits 40S and 60S, as well as monosomes (single ribosomes) and polysomes of increasing molecular weight is indicated. (B) Raji and SUDHL-4 cells were treated with ethanol (0 or 20 mM) for 10 days and then incubated for 20 minutes with 35S-labeled amino acids. Lysate aliquots of 50 μg of total protein were fractionated by SDS-PAGE, transferred onto PVDF membranes, and visualized and quantified using a PhosphorImager. Two independent experiments are shown.$

Chronic ethanol treatment leads to a suppression of global translation. (A) Raji and MCF-7 cells were treated with ethanol (0 or 20 mM) for 10 days. On the 10th day, cytoplasmic lysates were prepared and fractionated over sucrose gradients. From left to right, the distribution of free mRNA (not bound to ribosomes or ribosome subunits), ribosome subunits 40S and 60S, as well as monosomes (single ribosomes) and polysomes of increasing molecular weight is indicated. (B) Raji and SUDHL-4 cells were treated with ethanol (0 or 20 mM) for 10 days and then incubated for 20 minutes with ³⁵S-labeled amino acids. Lysate aliquots of 50 μg of total protein were fractionated by SDS-PAGE, transferred onto PVDF membranes, and visualized and quantified using a PhosphorImager. Two independent experiments are shown.

This Feature Is Available To Subscribers Only