Chronic ethanol treatment demonstrates significant antilymphoma activity in a human xenograft mouse model. Twenty-one mice were divided into 3 groups (control vs 5% EtOH vol/vol vs 10% EtOH vol/vol) with 7 mice each. Mice were injected subcutaneously with SUDHL-4 cells and continued on ethanol treatment for remainder of experiment. (A) Representative mice (mice 2, 5, and 8 from Western blots) were photographed before death to visually demonstrate tumor growth. (B) The average tumor volume of each group (n = 7) with SD is shown as a function of time (*P ≤ 2.17 × 10−5). (C) Xenograft tumors were excised from mice for further analysis. Tumors from control (1-3) 5% EtOH (4-6) and 10% EtOH (7-9) were lysated to obtain protein extracts. A total of 50 μg protein lysates was analyzed by Western blot and immunoblotted for pmTOR (Ser 2448), mTOR, pRPS6, RPS6, p-p70S6K, p70S6K, eIF4E, and β-actin. (D) Immunohistochemistry of paraffin-embedded xenograft tumors, excised from mice treated with ethanol (control vs 5% EtOH vol/vol vs 10% EtOH vol/vol), anti-pmTOR, and anti-pRPS6 antibody.
