Adenosine-differentiated DCs have up-regulated expression of pro-inflammatory COX-2, tolerogenic TGF-β and IDO, and tumor growth–promoting ARG2. (A-D) Immature or mature DCs were generated from human monocytes without or with 100 μM NECA, and total RNA was purified from cells as described in the “Methods.” Specific mRNA transcripts for indicated proteins were quantified by real-time PCR. Average values from 3 different experiments are shown. Error bars denote SE.