Reconstruction of Scl-centric regulatory networks. (A) In vivo validated core network. Blue arrows indicate activation through Scl-bound enhancers validated in transgenic mice. Red lines indicate protein-protein interactions curated from the literature.^58,–60  (B) Motif overrepresentation analysis of 228 Scl-bound regions shows overrepresentation of multiple consensus binding sites associated with stem cell regulators (for full results, see Table S2). (C) Extended Scl-centric TF network with Scl at the top and Scl targets involved in transcription regulation underneath. Red arrows correspond to Scl controlling target genes through elements found to be occupied by Scl in the current study. Gray arrows indicate inferred links based on the presence of overrepresented motifs from (B). Factors with inferred links are shown in purple if they are Scl targets, in green if they are not.