PRAME CTLs can be generated from HLA-A*02⁺ CML patients. (A) Frequency of IFNγ CTLs specific for ALY (top graph), SLY (middle graph), and VLD peptides (bottom graph) in 6 HLA-A*02⁺ CML patients. (B) PRAME CTLs produced IFNγ⁺ SFC in response to PRAME peptides and to autologous CML blasts. In addition, CTLs reacting against the ELA peptide (■) generated from the same patient did not produce IFNγ⁺ SFC when cocultured with autologous CML blasts. (C) PRAME CTLs produced IFNγ in response to autologous CML blasts but not to CML blasts from a PRAME⁺/HLA-A*02⁻ patient (patient 8).