Effect of LBH on survival, proliferation, and histone acetylation in diffuse large B-cell lymphoma cells. (A) DHL-6, Ly7, and Ly3 cells were treated with indicated concentrations of LBH589 for 24 hours. Western blot analysis of acetylated histones H3 and H4 were performed using specific antibodies. (B-C) DHL-6 and Ly7 diffuse large B-cell lymphoma (DLBCL) cells were treated with indicated concentrations of LBH589 for 48 hours and survival was assessed by annexin V/PI staining by flow cytometry. Data from 1 representative experiment are shown for each cell line (n = 3). (D) Primary cells from DLBCL patients were treated with various doses of LBH589 and survival was assessed by annexin V/PI staining. (E) LY7 cells were pretreated with 50 μM Z-VAD-fmk alone or with LBH589 (50 nM) for 48 hours, and survival was assessed by annexin V/PI staining. (F-G) DHL-6 and Ly7 cells were treated with indicated doses of LBH589 for 48 hours and cell proliferation was assessed by 3H-thymidine incorporation. Error bars indicate mean ± SD from 3 determinations.
