Removal of tissue-associated KC and MIP-2 is impeded in endotoxemic Sdc1^−/− mice. (A) WT and Sdc1^−/− mice were injected with LPS, and their lungs and livers were harvested at 7 or 30 hours after LPS. Tissues were weighed and homogenized, and tissue levels of TNFα, IL-6, KC, and MIP-2 were determined by ELISA (n = 5; *P < .05 relative to WT mice at the indicated time). (B) Total RNA was isolated from WT and Sdc1^−/− lungs at 0, 15, and 48 hours after LPS infusion, and KC, MIP-2, and β-actin mRNA was assessed by reverse transcription polymerase chain reaction. (C) WT or Sdc1^−/− splenocytes were stimulated with 100 ng LPS/mL for 24 hours at 37°C, and the concentration of TNFα, IL-6, KC, and MIP-2 in the conditioned medium was determined by ELISA (n = 4). Error bars indicate SE.