Resolution of neutrophilic inflammation is inhibited in endotoxemic Sdc1^−/− mice. (A) WT and Sdc1^−/− mice were injected with LPS, and their lungs, livers, and kidneys were isolated at 15 or 48 hours after LPS. Tissue sections were immunostained with the rat anti–mouse GR-1 (Ly6G/C, clone RBC6-8C5) antibody and Alexa 594 donkey anti–rat IgG antibody (original magnification, ×200). Similar results were obtained with the rat anti–mouse Ly6G (1A8) monoclonal antibody (not shown). (B) WT and Sdc1^−/− lung sections (24 hours after LPS) were coimmunostained with rabbit anti–cleaved caspase-3 and anti-GR1 antibodies and with Alexa 594 anti–rat and Alexa 488 goat anti–rabbit antibodies (original magnification, ×200). (C) WT and Sdc1^−/− lung sections (24 hours after LPS) were immunostained with rat anti-CD14 and Alexa 594 anti–rat antibodies (original magnification, ×200).