Gli1^null KSL cells have increased engraftment after transplantation due to increased numbers of LT-HSCs. (A) Gli1 (β-Galactosidase) and Ptch expression levels by q-PCR in LT-HSCs (CD34^negcKit⁺Sca1⁺Lin^neg), ST-HSCs (CD34⁺cKit⁺Sca1⁺Lin^neg), CMPs (FcRγ^lowCD34⁺cKit⁺Sca1^negLin^neg), and GMPs (FcRγ^hiCD34⁺cKit⁺Sca1^negLin^neg) from Gli1^LacZ/wt mice. (B) Five hundred KSL cells from Gli1^wt and Gli1^null male mice with 250 000 female competitor bone marrow cells were transplanted into irradiated female hosts. Peripheral blood chimerism of individual mice at 16 weeks after transplantation is indicated by diamonds and the average chimerism, by a horizontal bar. *P < .05. (C) Whole bone marrow cells were labeled with fluorescent-labeled antibodies and analyzed by flow cytometry for the LT-HSC (CD34^negFlt3^negKSL), ST-HSC (CD34⁺Flt3^negKSL), and MPP (CD34⁺Flt3⁺KSL) fractions within the KSL compartment. Representative results from one pair of mice are shown. Percentages are of the KSL fraction. (D) Results for KSL subsets are summarized. Results are mean percentage of total bone marrow mononuclear cells ± SEM from 3 individual mice. *P < .05.