Loss of Gli1 impairs stress hematopoiesis. (A) Two cohorts of mice (total of 10 mice/group) were treated with 5-FU (150 mg/kg) and neutrophil counts measured. Each time point represents the mean ± SEM from 4 to 5 mice. (B) Mice were treated with 5-FU (150 mg/kg) and platelet counts measured. Each time point is the mean ± SEM from 5 mice. (C) Mice were treated with G-CSF (10 mg/kg) intraperitoneally daily for 3 days, and neutrophil counts measured after 3 days. Results are mean ± SEM from 5 mice. *P < .05.