Tissue-specific GVHD caused by Th1, Th2, and Th17 cells was associated with tissue-specific T-cell migration. (A) Yield of H-2b⁺CD4⁺ T cells in spleen or tissues of recipients given WT or IFN-γ^−/− donor cells 13 days after HCT. Mean ± SE is shown (n = 8), combined from 3 replicated experiments. (B-C) Five days after HCT, splenocytes from recipients were stained for H-2^b, CD4, and chemokine receptors. Gated H-2b⁺CD4⁺ T cells are shown in CD4 versus chemokine receptors. Representative of 4 replicate experiments is shown. (D-E) Mean ± SE of chemokine expression levels by GVHD target tissues 5 days after HCT (n = 4). Relative gene expression levels were normalized within each sample to housekeeping gene GAPDH. Data are a representative of 2 replicated experiments.