BCL6 target genes display specific genomic features and DNA elements. (A) The diagram depicts the overlap between the overlapping BCL6 genes between normal and malignant B cells (blue circle) and CpG island-containing loci among the entire pool of 24 137 promoters contained on the array. (B) Analysis of the BCL6 target gene cohort by FIRE identified 8 DNA elements significantly overrepresented among BCL6 target genes identified in GC cells (CB), DLBCL cells, the common target genes, or in the cohort of non-BCL6 targets (columns). Yellow represents highly enriched; and blue, relative depletion. The statistical significance of the heatmap color code is provided. (C) The graph represents the distribution of genes ordered according to the strength of BCL6 binding in ChIP-on-chip on the x-axis and the frequency with which genes contain the BCL6 consensus site identified by FIRE (inset), using a sliding window of 100 genes. (D) Genes are distributed along the x-axis according to BCL6 binding, and the y-axis depicts the position of the FIRE-identified BCL6 motif occurrences relative to the transcriptional start site (set at 0). Negative numbers are upstream and positive numbers downstream of the start site. Similar to panel C, the y-axis corresponds to the average position of BCL6 motif occurrences with respect to the transcription start site using a sliding window of 100 genes. Only genes containing at least one occurrence of the BCL6 motif were considered. (E) Similar to panel C, the graph represents BCL6 binding on the x-axis, but the y-axis shows the frequency at which genes contain both BCL6 and STAT3-binding elements.