Figure 2
Agonist-induced platelet ATP secretion and thromboxane B2 synthesis. (A) TxB2 was measured by ELISA in buffer containing washed platelets from subject P1 and from a healthy experimental control at baseline and after incubation with 1μM AA. The results are from 1 experiment performed in triplicate. (B) ATP secretion in response to the indicated concentrations of AA, U46619, and CRP was determined by measuring luminescence signal from PRP in the presence of luciferase in a Chronolog lumiaggregometer. (C) ATP secretion in response to 3μM U46619 in washed platelets from subject P2, who had the same variant D304N TxA2R as P1. Data are expressed as concentration of ATP in PRP. The results are representative of 2 experiments.
