Specific cell-surface binding of [125I]-rHuEpo was detected in 1 of 5 of the higher EpoR protein–producing cell lines. Specific binding of [125I]-rHuEpo to live intact cells (1 × 106 cells) is expressed as percentage of control UT-7/Epo cells and is the average of 2 to 3 experiments (N = 5 samples/cell for each experiment). The negative control was 769-P cells. Error bars represent SD. Note specific binding with NCI-H661 cells (P ≤ .001).