Western blots of EpoR knockdown for 8 different siRNAs. A82 Western blot of siRNA mediated knockdown of EPOR in transfected cells with 8 EPOR siRNAs, 2 nontargeting control siRNAs (NT), 2 cyclophilin A siRNAs (PPIA-1, PPIA-2), and an untransfected control (UT). CycloB is a loading control. Blots are representative of experiments performed on lysates from duplicate transfections on separate days. (A) UT-7/GM-CSF cells showing decreased EpoR protein in response to EPOR siRNA treatments. In the UT-7/Epo cells, there was a direct correlation between the degree of cell death observed (Figure 6) and the degree of EpoR knockdown with the 8 siRNAs (R2 = .6973; supplemental Figure 4). (B) A2780 cells and (C) NCI-H1299 cells both grown in rHuEpo show a decrease in EpoR protein in response to siRNA treatments.
