Determination of Fn in plasma and platelets by Western blot analysis. (A) Plasma Fn was depleted in Cre⁺ TKO mice but not altered in the plasma of Cre⁻ littermates after polyI-polyC treatment. (B) Platelet Fn content in Cre⁺ TKO mice was also markedly decreased compared with Cre⁻ control platelets. Vinculin is shown as an internal loading control.