Platelet surface expression of adhesion molecules and integrin activation is similar between Cre⁺ TKO and Cre⁻ control platelets. (A) Platelet surface expression of adhesion molecules was detected by flow cytometry. GPIbα, β₃ integrin (CD61) and β₁ integrin (CD29) on Cre⁺ TKO platelets (gray line) and Cre⁻ control platelets (black line) were analyzed. Negative controls are shown as filled peak. For P-selectin (CD62P) expression, resting platelets (filled peak) or thrombin (1 U/mL)–stimulated Cre⁺ TKO (gray line) and Cre⁻ (black line) platelets were analyzed. No difference in surface expression was observed. (B) β₃ Integrin activation on Cre⁺ TKO platelets (gray line) and Cre⁻ control platelets (black line) by thrombin (1 U/mL) and ADP (20 μM) were tested with the use of JON/A-PE antibody. There were no detectible differences in β₃ integrin activation between the 2 groups of platelets. Representative data are shown from 6 independent experiments.