Amplification plots for mRNA for PGE₂ receptors. (A) Primers designed to specifically detect murine EP1, EP2, EP3, or EP4 were used for QRT-PCR (with SYBR green) and plots with an activation step of 50°C for 2 minutes, denaturation at 95°C for 2 minutes, and amplification for 45 cycles at 95°C for 15 seconds, 50°C for 30 seconds, and 72°C for 30 seconds are shown. Plots corresponding to specific EP receptors are indicated in each amplification plot, where the legend key on the right shows the relative order of transcripts top to bottom. *Denotes the presence of at least 2 dissociation peaks indicating the presence of splice variants. (B) EP receptor amplification on human UCB CD34⁺ and CD34⁺ CD38⁻ cells with the same QRT-PCR procedure as described for panel A.