TCR signal strength regulates cytokine-dependent T_CM development. CD8⁺ CD45RA⁺ sorted cells were labeled with PBSE and polarized under either neutralizing or IL-12 + IFN-α conditions with 1 μg/mL, 1.5 μg/mL, 2.5 μg/mL, or 5 μg/mL anti-CD3/anti-CD28 for 5 days. (A) Assessment of division by PBSE dilution as a function of primary activation strength. The percentages of cells that are contained within each gate are indicated above the gate. (B) Cells were analyzed for expression of CXCR3 and CCR7 by bivariant dot plot analysis.