Kinetics of surface markers and cytokine expression in alloreactive T cells (n = 5). FACS analysis of expression of surface markers and intracellular cytokines in CFSE labeled T cells cocultured with HLA-mismatched DCs. Results are means ± SD. Unstimulated control data has been subtracted from the stimulated data. Because CCR7 and CD45RA showed high expression in unstimulated PBMCs, they are not shown