Akt2 is important for neutrophil release of β-glucuronidase. Bone marrow–derived neutrophils from wild-type (WT), Akt1^−/−, and Akt2^−/− mice were preincubated without (A) or with (B) cytochalasin B (10μM) for 15 minutes on ice and 15 minutes at 37°C. The cells were then stimulated with fMLF at indicated concentrations for 10 minutes. The released β-glucuronidase was quantified and expressed as percentage of total cellular β-glucuronidase. The results are shown as means ± SEM, on the basis of 3 independent experiments each conducted in duplicate. *P < .05, **P < .01, compared with WT in the same stimulation group.