Deficiency of Akt2 but not Akt1 impairs neutrophil migration. Bone marrow–derived neutrophils from WT, Akt1^−/−, and Akt2^−/− mice were seeded onto glass coverslips coated with fibrinogen and stimulated with 1μM fMLF. The migration of the neutrophils under a uniform fMLF concentration was monitored periodically, and video images were recorded. The path of cell migration over time is shown in the bottom panels. Representative images, collected from 3 independent experiments, are shown in panel A. In panel B, the distance traveled by the cells was calculated by use of the ImageJ software with manual tracking (n = 10 from each sample). *P < .05 compared with WT mouse neutrophils. Video clips for cell migration are shown in the supplemental Videos.