Lin⁻CD117⁺ BM cells from TSC-22^−/− mice show higher proliferation than those from TSC-22^+/+ mice in vivo. (A) Representative example of BM cells obtained from TSC-22^+/+ and TSC-22^−/− mice after ingesting BrdU-containing drinking water for 7 consecutive days. BM cells were stained for Lin markers, CD117, and BrdU and then were analyzed by flow cytometry. The region shaded in gray represents Lin⁻CD117⁺ BM cells from TSC-22^−/− mice and shows higher BrdU incorporation than identical BM cells obtained from TSC-22^+/+ mice (open area, black line). (B) Summary of data represented in panel A but performed on 3 pairs of littermate mice. There is significantly higher BrdU incorporation in the TSC-22^−/− Lin⁻CD117⁺ BM cells compared with those of TSC-22^+/+ mice (P < .05, n = 3). Error bars indicate standard errors for 3 independent experiments.