MiR-146a is expressed in T lymphocytes. (A) MiR-146a levels were measured in human purified primary CD4⁺ and CD8⁺ cells by qRT-PCR, using U6 levels to normalize expression. (B) CD4⁺CD45RA⁺ human naive T lymphocytes were stimulated with PMA and ionomycin and miR-146a levels were measured at different times by qRT-PCR. (C) Jurkat cells were stimulated with anti-CD3/CD28 Abs or with PMA and ionomycin and MiR-146a levels were measured at different times by qRT-PCR. Each bar is the mean of 3 independent experiments.