American Society of Hematology

Figure 5

$Figure 5. MiR-146a overexpression inhibits the AICD of Jurkat T cells. (A) pRRL-ct and pRRL-146a Jurkat cells, plated in RPMI medium with 3% FBS, were left untreated (control) or stimulated with anti-CD3 (OKT3; 10 μg/mL) Ab. After 24 hours the apoptotic cells fraction was measured by annexin V staining. Results shown are 1 representative of 3 independent experiments. (B) pRRL-ct and pRRL-146a Jurkat cells, plated in RPMI medium with 3% FBS, were left untreated (control) or stimulated with Fas-agonistic Ab (CH11 clone; 100 ng/mL) for 6 hours or (C) for 24 hours. The apoptotic cells fraction was measured by annexin V and propidium iodide double staining. Results shown are 1 representative of 3 independent experiments.$

MiR-146a overexpression inhibits the AICD of Jurkat T cells. (A) pRRL-ct and pRRL-146a Jurkat cells, plated in RPMI medium with 3% FBS, were left untreated (control) or stimulated with anti-CD3 (OKT3; 10 μg/mL) Ab. After 24 hours the apoptotic cells fraction was measured by annexin V staining. Results shown are 1 representative of 3 independent experiments. (B) pRRL-ct and pRRL-146a Jurkat cells, plated in RPMI medium with 3% FBS, were left untreated (control) or stimulated with Fas-agonistic Ab (CH11 clone; 100 ng/mL) for 6 hours or (C) for 24 hours. The apoptotic cells fraction was measured by annexin V and propidium iodide double staining. Results shown are 1 representative of 3 independent experiments.

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