FADD is a target of miR-146a. (A) The FADD-3′UTR luciferase construct was cotransfected in 293T cells with miR-146a mimic or with a negative control mimic (scr mimic). Results are expressed as mean (±SD, n = 3) of the ratio between renilla luciferase and firefly control luciferase activities (*P < .01). (B) Intracellular staining and FACS analysis for FADD protein. FADD protein levels were measured as mean fluorescence intensity (MFI). Values of MFI are expressed as mean (±SD, n = 7). Black histogram represents pRRL-ct Jurkat cells stained with FADD Ab, gray histogram represents pRRL-146a Jurkat cells stained with FADD Ab, and dashed open line represents pRRL-ct and pRRL-146a Jurkat cells stained with anti–rabbit fluorescein isothiocyanate (FITC) secondary antibody alone.