Mechanism underlying the antiangiogenic function of HDAC5. (A) Scheme of different HDAC5 constructs. (B) RT-PCR analysis of HUVECs overexpressing different HDAC5 constructs after 14 hours. (C) Western blot analysis of HUVECs overexpressing different HDAC5 constructs after 14 hours. (D) Capillary-like sprout formation from spheroid cultures of HUVECs overexpressing different HDAC5 constructs (n = 3-10; *P < .05 vs mock). (E) Lysates of HUVECs overexpressing different HDAC5 constructs were immunoprecipitated with an antibody against c-myc. Western blots were performed with an anti-c-myc or an anti-MEF2 antibody in whole cell lysates or after immunoprecipitation. Ab indicates antibody control.