American Society of Hematology

Figure 4

$Figure 4. Photomicrographs showing that leukemia cells show a reduced mitotic arrest after ICRF-193 and restoration of mitotic arrest with reduced Metnase. (A) Images showing both tubulin (green) and MPM-2 (red) staining, as well as counterstaining with DAPI, after 10 μM ICRF treatment for 24 hours. There are more cells arrested in mitosis after ICRF-193 in the CD34+ cells than in the THP-1 AML cells. When Metnase is repressed in the THP-1 cells, the fraction of cells in mitosis after ICRF-193 exposure becomes similar to that seen with CD34+ cells. (B) Similar to panel A, but treated with 25 μM ICRF for 24 hours.$

Photomicrographs showing that leukemia cells show a reduced mitotic arrest after ICRF-193 and restoration of mitotic arrest with reduced Metnase. (A) Images showing both tubulin (green) and MPM-2 (red) staining, as well as counterstaining with DAPI, after 10 μM ICRF treatment for 24 hours. There are more cells arrested in mitosis after ICRF-193 in the CD34⁺ cells than in the THP-1 AML cells. When Metnase is repressed in the THP-1 cells, the fraction of cells in mitosis after ICRF-193 exposure becomes similar to that seen with CD34⁺ cells. (B) Similar to panel A, but treated with 25 μM ICRF for 24 hours.

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