Effects of ADAMTS-13 concentration. Time-to-rupture t₁₁ (A), time-to-destabilization t₂₁ (B), and time-to-rupture t₂₂ of the short (C) and long (D) groups were plotted versus ADAMTS-13 concentration. With the exception of t₂₂, which was shortened by ADAMTS-13 in a dose-dependent manner, other time parameters were indifferent to the changing ADAMTS-13 concentration. DisC is a control construct without VWF-cleaving activity. Data were acquired by GPIbα-pulling and presented as mean ± SEM of several tens of measurements.