Effects of whole plasma on in vitro megakaryocyte production, CFU-MK, platelet release, and megakaryocyte apoptosis. (A) ITP plasma of group A significantly increased the yield of megakaryocytes on day 15 compared with control cultures (7.13 ± 0.86 × 105 vs 5.10 ± 0.90 × 105, P < .0001). ITP plasma of group B significantly reduced the yield of megakaryocytes on day 15 compared with control cultures (2.83 ± 1.02 × 105 vs 5.10 ± 0.90 × 105, P < .0001). Significant difference in megakaryocyte counts was not seen between group C and control cultures (5.20 ± 0.66 × 105 vs 5.10 ± 0.90 × 105, P = .776). (B) In the presence of whole plasma, in vitro CFU-MK formation in groups A, B, C, and controls were 11.89 ± 2.93, 11.50 ± 2.62, 10.56 ± 2.35, and 12.05 ± 2.72, respectively. There was no remarkable differences among the 4 groups (P = .553). (C) In the presence of whole plasma, platelet release in group A was significantly lower than controls (7.51 ± 2.41 × 103 vs 11.21 ± 1.82 × 103, P < .0001). Platelet release in group B was significantly lower than controls (7.21 ± 2.45 × 103 vs 11.21 ± 1.82 × 103, P < .0001). There was no remarkable differences between group C and controls (10.12 ± 1.91 × 103 vs 11.21 ± 1.82 × 103, P = .214). (D) In the presence of whole plasma, group A cultures showed decreased apoptotic megakaryocytes (21.88% ± 3.53%) compared with group B (27.36% ± 4.31%), group C (28.21% ± 4.02%) and control cultures (29.43% ± 3.80%, P < .0001). Data are mean ± SD.
