Effects of antibody-adsorbed plasma on in vitro megakaryocyte production, CFU-MK, platelet release, and megakaryocyte apoptosis. (A) After adsorption of autoantibody from patient plasma, megakaryocyte number rose to control level, and even more megakaryocytes were present in cultures with adsorbed group A plasma than those cultured with control plasma (7.85 ± 1.30 × 105, 5.29 ± 0.74 × 105, P < .0001). (B) After antibody adsorption, significant difference in CFU-MK was not seen among groups A, B, C, and controls (P = .816). (C) After adsorption, significant difference in platelet release was not seen among groups A, B, C, and controls (P = .061). (D) After adsorption, megakaryocyte apoptosis in group A was still lower than that in controls (22.44% ± 3.56% vs 30.24% ± 3.96%, P < .0001). All data were presented as mean ± SD.
