Specificity of CB CTL lines for adenovirus hexon. CB-derived CTL lines were screened by ELISPOT assay against 11 pools of hexon peptides (20mers overlapping by 15 aa) to identify which peptide pools were being recognized by each CTL line. (A) The CTL line CB6 showed specific IFN-γ release specifically against peptide pool 1. (B) The CTL line CB9 showed specific IFN-γ release specifically against peptide pool 10. Results are expressed as spot-forming cells (SFCs)/105 CTLs. (C) To identify the stimulating 20mer peptides, CTL line CB6 was rescreened against the individual peptides contained in pool 1. The CTL reactivity against pool 1 were mapped to the overlapping peptides 15 (VDREDTAYSYKARFTLAVGD) and 16 (TAYSYKARFTLAVGDNRVLD). (D) In contrast, the reactivity in CTL line CB9 was mapped to peptides 6 (MYSFFRNFQPMSRQVVDDTK) and 7 (RNFQPMSRQVVDDTKYKDYQ) in pool 10. Results are expressed as SFCs per 105 CTLs.
