BCOR-RARA associates with the RARA responsive element with RXRA. (A) 35S-Methionine–labeled input proteins. (B) EMSA analysis with in vitro translated proteins and radiolabeled DR5G RARE probes. Without RXRA, PML-RARA associated with RARE, but BCOR-RARA did not. BCOR and BCOR-S did not associate with RARE, regardless of the presence of RXRA. 100× competitor: 100-fold excess of nonradiolabeled-RARE was additionally incubated with the reaction. (C) CBC-RARA did not associate with RARE without RXRA. In addition, 4-fold increased input proteins in less in vitro translation kit solution were subjected to EMSA to enhance specific bands and reduce nonspecific background. An arrow indicates an input protein/RARE complex included in the upper lane. Control Ab, normal rabbit polyclonal IgG; N.S., nonspecific band.
