Characterization of primary BM hematopoietic cell–derived iPSCs generated using the 4 iPSC factors. (A) Reverse-transcription PCR analysis showing ES marker gene expression in primary BM HSPC-derived iPSC clones (pHPC-iPSCs). H2O indicates no-template control; ES, ES cells as a positive control; RT (−), no-reverse-transcriptase control. A vertical line has been inserted to indicate a repositioned gel lane. (B) PCR analysis for Ig gene rearrangement of D-J segments (DJ1-DJ3) in pHPC-iPSC clones. GL indicates amplification of the fragment representing unrearranged, germline configuration of the Ig heavy chain gene; EL4, a T lymphoma cell line as an unrearranged control. (C) Histologic sections of teratomas derived from a pHPC-iPSC clone. (D) Images of pHPC-iPSC colonies derived from an EGFP-transgenic mouse. Bars represent 100 μm (C-D). (E) E10.5 chimeric embryos generated with one representative EGFP+ iPSC clone.
