Analysis of hematopoietic cells from gata2^eGFP transgenic fish by flow cytometry. Contour plots show the regional separation of major blood cell lineages by their light-scatter characteristics: FSC^loSSC^int-hi (R1, erythroid gate), FSC^intSSC^lo (R2, lymphoid gate), FSC^hiSSC^lo (R3, precursor gate), FSC^hiSSC^int (R4, myeloid gate), and FSC^hiSSC^hi (R5, eosinophil gate). Histograms indicate the abundance of GFP⁺ cells in each gate. (A) A representative scatter profile for WKM. Cells within each gate were quantified using the mean ± SD: R1, 37% ± 8.1%; R2, 17% ± 3.95%; R3, 6% ± 1.4%; R4, 24% ± 6.9%; and R5, 3% ± 1.3%. gata2^hi cells were only present in the R5 gate: 74% ± 10%; n = 26. (B) A representative scatter profile for IPEX: R1, 9% ± 4.9%; R2, 10% ± 5.2%; R4', 7% ± 2.4%; and R5, 46% ± 18%. gata2^hi cells were only present in the R5 gate: 91% ± 9.5%; n = 16.