IFN-α and TLR-related antiviral molecules by microarray. Monocyte-derived macrophages (n = 3) were stimulated with IFN-α (10 ng/mL, 4 hours) in parallel with mock-treated cultures (see supplemental Table 1) and with LPS-stimulated cultures (100 ng/mL)27 and RNA extracted and processed for microarray analysis using the Affymetrix system. Macrophage gene expression after treatment with IFN-α as ratio to control (IFN-α/c) compared with LPS/Control ratio, in common logartithmic scale. Only probe sets of suspected antiviral functions are shown. The line of identity is shown to highlight the points that are similar in both measures. Density contours are shown for the entire set of 54 675 probe sets on the Affymetrix HG_U133 Plus2 chip. Ratios shown in common logarithmic scale. SFC = S10 transform fold change.
