MDS/MPN phenotype in lyn^−/−;PLC-β3^−/− mice. (A) Flow cytometric analysis of nucleated cells in BM, spleen (SPL), and peripheral blood leukocytes (PBL) at the age of 3 months. (B) Graphic summary of granulocytes and monocytes (CD11b⁺ cells) in these organs of 2- to 4-month-old lyn^−/−;PLC-β3^−/− mice (n = 12 per cohort). Data are mean ± SD, *P < .05. (C) Spleens at the age of 4 months. (D) Mature granulocytes and monocytes in blood smear at the age of 4 months. (E) Hematoxylin and eosin staining of monolobated megakaryocytes in the BM of lyn^−/−;PLC-β3^−/− mice, compared with multilobated ones in wt mice. (F) Graphic summary of megakaryocytes with the indicated numbers of nuclear lobes in BM at the age of 4-6 months (n = 3).