Phenotypic similarities between lyn^−/−;PLC-β3^−/− and me^v/me^v mice. (A) Flow cytometric analysis of granulocytes and monocytes in BM and peripheral blood from 6-week-old me^v/me^v mice. (B) Immunofluorescent staining for F4/80 and Ym-1 in lung tissues. Bar indicates 50 μm. (C) BM cells were subjected to flow cytometric analysis for HSCs/progenitors. The absolute number was calculated based on the percentage of KSL cells in BM. (D) BM cells were stimulated with IL-3 and then phospho-Stat5 levels in KSL cells were analyzed by flow cytometry (n = 4). (E) Sorted CD34⁻KSL cells (50 cells/well) were cultured in 96-well plates in the presence of IL-3 and SCF. Data are mean ± SD, *P < .05.