Immune priming in the spleen of wild-type and CCX-CKR^−/− mice. (A) Total cell numbers and CD4⁺ cell numbers from wild-type and CCX-CKR^−/− spleens on day 12 postimmunization for EAE. Data shown are the mean ± SEM (*P < .05). (B) Six mice per group were immunized for EAE and fed BrdU at 0.8 mg/mL in their drinking water from day 6. On day 9, draining LNs and spleens were harvested, stained for CD4⁺ and BrdU, and analyzed by flow cytometry. Representative dot plots are shown. Data are the mean proportion of CD4⁺/BrdU⁺ cells ± SEM. (C) On day 12 postimmunization for EAE, spleens were harvested, red blood cells lysed, viable cells counted, and cells cultured overnight at 4 × 10⁶/mL in complete RPMI at 37°C, 5% CO₂. Cells were then harvested and stained for flow cytometric analysis. Data shown are from 11 mice per group in total from 2 independent experiments pooled and represent the mean ± SEM (**P < .01). Representative dot plots showing the gating for CD4 and CCR6 are shown.