CTI-inhibited fresh blood activated with rTf or LPS-THP-1 in the absence of EA.hy926. Bare tubes containing rTf (■) or LPS-THP-1 (♦) activated whole blood was used to measure (A) TAT formation over time, (B) prothrombin consumption, (C) FPA production, and (D) fVa generation (left), inactivation (right), and representative Western blot. FVa_1-506 HC cleavage is also shown but was not quantified. Clot times in all experiments are indicated (■ = 3.0 ± 0.17 minutes and ♦ = 3.0 ± 0.34 minutes). Dashed line in panels A and C represents 95% confidence limits.