Longer lasting effect of SAnxA1 on leukocyte adhesion in postcapillary venules. Mice were injected intrascrotally with either saline or 50 ng IL-1β, and the extent of leukocyte rolling and adhesion was quantified 3 hours later. Image is representative of the inflammatory status induced by the cytokine. Data are mean ± SEM of 6 mice per group. *P < .01 versus vehicle. Mice pretreated with IL-1β were instrumented an administered via the jugular vein with PBS, AnxA1 (0.6 mg/kg), or SAnxA1 (0.6 mg/kg), and the microcirculation monitored for further 30 minutes both for cell rolling velocity (B) and cell adhesion (C). Values are mean ± SEM of 5 mice per group. *P < .01 versus vehicle group and #P < .05 versus respective AnxA1 group. (D) FPR2 null mice were treated with IL-1β and recombinant proteins as described in panels B-C. Values are mean ± SEM of 5 mice per group.