Monocyte-mediated trogocytosis of RBCs sensitized with e-IgA autoantibodies. PKH-67–labeled RBCs were incubated with PBMCs for 90 minutes. Spontaneous membrane transfer between RBCs and various leukocyte populations (see the gates; column I), before (T0) and after coculture (T90) are visualized as an acquisition of PKH67 mfi by: CD4⁺ (line I), CD8⁺ (line II) T lymphocytes; CD56⁺ NK cells (line III) and CD14⁺ monocytes (line IV), respectively. RBCs were sensitized, or not (columns I and II, respectively), with a mAb directed against RhD (mIgG anti-RhD; column III), a polyclonal IgG against RhD (Rhophylac, column IV) or with eluted IgA autoantibodies (e-IgA; column V). Sensitization with mIgG anti-RhD and Rhophylac served as positive controls. Data were collected on a BD FACScan cytometer and analyzed with CellQuest Pro software Version 4.0. Dot plots show the results from one representative experiment. The numbers indicated represent the mfi of the gated populations. Similar results were obtained in 2 other independent experiments. We visualized and analyzed at least 3000 gated events for each cell population.